(4) Results and analysis
What parameters am I measuring?
Here are several standard parameters to quantify water cleanliness:
- TCC Total Cell Count /mL (count of all points within the gate): Current WHO guidelines stipulate a count of zero TTC bacteria for ingested water
- ICC Intact Cell Count
- HNAC High Nucleic Acid Count /mL
- LNAC Low Nucleic Acid Count /mL
- HNAP High Nucleic Acid Percentage = The HNAP (%) is the High nucleic acid percentage = HNAC/TCC × 100%.
With the sample analysis, every cell generates signal peaks on the 3 channels: SSc, FL1 and FL2. The signal peaks of FL1 and FL2 are represented by one dot in the dot plot, as shown on figure 6.
Finally, a cytogram is generated by the data processing: The TCC (total cell count) value is the count of all points within the gate. The LNAC/mL (low nucleic acid count) is the count of all points in the “right” gate compartment. The HNAC/mL (high nucleic acid count) is the count of all points in the “left” gate compartment. The detection limit is 100 cells/mL- 5000000 cells/mL.
The results are available within 20 minutes. A large intuitive touch screen serves as user interface. The measuring interval and further settings can be programmed simply and quickly thanks to the embedded software.
6 & 7 Waste management and cleaning
The generated waste products are enclosed in the sealed cartridge, which allows for safe handling and easy disposal. The system is cleaned and rinsed. This involves waste generation and removal. This reduces sample contamination and the effect on subsequent measurements.