Combining an SPM and an RVM, it is possible to create an automatic cell culture media management system.
At the input, different medias or components of medias are connected, so as to test the perfect media balance for your cells, or simply to renew/change the media automatically.
At the output, the media is either sent to waste, or through an analyser. This can be used as a simple monitor or, with feeback from the analyser, it could make an automatic system to have the perfect conditions for your cell growth at all times!
Many different parameters can be monitored in cell culture media, to guarantee the best growing parameters, or define the time to change the cells, such as: pH, amino acids, sugars, vitamins, nucleic acid components etc.
In this setup, it has been chosen to monitor 4 parameters: pH, oygen, glucose and ammonium.The goal values for each of these parameters depends on the cell that is being grown. Nevertheless, here is the order of magnitude for mammalian cells for each parameter:
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